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celltag v1 plasmid library  (Addgene inc)
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Addgene inc celltag v1 plasmid library
(A) Schematic displaying experimental design for lineage-tracing BTOs using CellTagging. BTOs were differentiated using the 21-day Airway Differentiation Paradigm (Fig S2A). At 21 days, Airway Organoids were transduced with the CellTagging lentiviral-based library of barcoded GFP plasmids. After 7 days, cells were sorted for GFP + and remaining BTPs were removed using CPM. Single clones were replated and grown for 30 days, then submitted for scRNA-sequencing. (B) UMAP feature plots of cell-type scoring annotation for predominant cell types on CellTagged-Airway Organoids. Cells most aligning to the scoring set are in orange, with little to no scoring represented in blue. (C) UMAP cluster plot of sequenced 58-day CellTagged-Airway Organoids. Each dot represents a single cell and cells were computational clustered based on transcriptional similarities. Clusters were colored and labeled by cell type, which were determined based on expression of ‘Cell Type Score’ ( , S2H, Table S2) derived from Fetal Epithelial dataset. (D) UMAP plots of CellTagged-Airway Organoids overlaid with cells labeled from FAS-Rooted or basal-rooted clones. (E) UMAP plot of FAS-rooted and basal-rooted clones overlaid. (F) Quantification of cell types within FAS- or basal-rooted clones. Number of cells per cell type were divided by total number of cells of -rooted clone sample to normalize and report as percentage. (G) UMAP cluster plot of multiciliated clone extraction from 58-day CellTagged-Airway Organoids. <t>CellTag-expressing</t> cells within the multiciliated cluster in were computationally extracted and sub-clustered resulting in 3 subclusters. Cells from FAS-rooted or basal-rooted clones are shown as red dots in top middle and right panels. Bottom panels are UMAP feature plots of multiciliated marker FOXJ1 , and enriched genes C6 and MUC16 . The color of each dot in the feature plot indicates log-normalized expression level of the genes in the represented cell. (H) Dot plot for expression of C6 and MUC16 between FAS-rooted and basal-rooted clones within multiciliated clone extraction . The dot size represents the percentage of cells expressing the gene in the corresponding cluster, and the dot color indicates log-normalized expression level of the gene.
Celltag V1 Plasmid Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltag+a+plasmid/CellTagA+(Plasmid+%23124591)/bio_rxiv__2022__06__13__495813-251-1-8
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celltag v1 plasmid library - by Bioz Stars, 2026-09
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(A) Schematic displaying experimental design for lineage-tracing BTOs using CellTagging. BTOs were differentiated using the 21-day Airway Differentiation Paradigm (Fig S2A). At 21 days, Airway Organoids were transduced with the CellTagging lentiviral-based library of barcoded GFP plasmids. After 7 days, cells were sorted for GFP + and remaining BTPs were removed using CPM. Single clones were replated and grown for 30 days, then submitted for scRNA-sequencing. (B) UMAP feature plots of cell-type scoring annotation for predominant cell types on CellTagged-Airway Organoids. Cells most aligning to the scoring set are in orange, with little to no scoring represented in blue. (C) UMAP cluster plot of sequenced 58-day CellTagged-Airway Organoids. Each dot represents a single cell and cells were computational clustered based on transcriptional similarities. Clusters were colored and labeled by cell type, which were determined based on expression of ‘Cell Type Score’ ( , S2H, Table S2) derived from Fetal Epithelial dataset. (D) UMAP plots of CellTagged-Airway Organoids overlaid with cells labeled from FAS-Rooted or basal-rooted clones. (E) UMAP plot of FAS-rooted and basal-rooted clones overlaid. (F) Quantification of cell types within FAS- or basal-rooted clones. Number of cells per cell type were divided by total number of cells of -rooted clone sample to normalize and report as percentage. (G) UMAP cluster plot of multiciliated clone extraction from 58-day CellTagged-Airway Organoids. CellTag-expressing cells within the multiciliated cluster in were computationally extracted and sub-clustered resulting in 3 subclusters. Cells from FAS-rooted or basal-rooted clones are shown as red dots in top middle and right panels. Bottom panels are UMAP feature plots of multiciliated marker FOXJ1 , and enriched genes C6 and MUC16 . The color of each dot in the feature plot indicates log-normalized expression level of the genes in the represented cell. (H) Dot plot for expression of C6 and MUC16 between FAS-rooted and basal-rooted clones within multiciliated clone extraction . The dot size represents the percentage of cells expressing the gene in the corresponding cluster, and the dot color indicates log-normalized expression level of the gene.

Journal: bioRxiv

Article Title: Distinct airway progenitor cells drive epithelial heterogeneity in the developing human lung

doi: 10.1101/2022.06.13.495813

Figure Lengend Snippet: (A) Schematic displaying experimental design for lineage-tracing BTOs using CellTagging. BTOs were differentiated using the 21-day Airway Differentiation Paradigm (Fig S2A). At 21 days, Airway Organoids were transduced with the CellTagging lentiviral-based library of barcoded GFP plasmids. After 7 days, cells were sorted for GFP + and remaining BTPs were removed using CPM. Single clones were replated and grown for 30 days, then submitted for scRNA-sequencing. (B) UMAP feature plots of cell-type scoring annotation for predominant cell types on CellTagged-Airway Organoids. Cells most aligning to the scoring set are in orange, with little to no scoring represented in blue. (C) UMAP cluster plot of sequenced 58-day CellTagged-Airway Organoids. Each dot represents a single cell and cells were computational clustered based on transcriptional similarities. Clusters were colored and labeled by cell type, which were determined based on expression of ‘Cell Type Score’ ( , S2H, Table S2) derived from Fetal Epithelial dataset. (D) UMAP plots of CellTagged-Airway Organoids overlaid with cells labeled from FAS-Rooted or basal-rooted clones. (E) UMAP plot of FAS-rooted and basal-rooted clones overlaid. (F) Quantification of cell types within FAS- or basal-rooted clones. Number of cells per cell type were divided by total number of cells of -rooted clone sample to normalize and report as percentage. (G) UMAP cluster plot of multiciliated clone extraction from 58-day CellTagged-Airway Organoids. CellTag-expressing cells within the multiciliated cluster in were computationally extracted and sub-clustered resulting in 3 subclusters. Cells from FAS-rooted or basal-rooted clones are shown as red dots in top middle and right panels. Bottom panels are UMAP feature plots of multiciliated marker FOXJ1 , and enriched genes C6 and MUC16 . The color of each dot in the feature plot indicates log-normalized expression level of the genes in the represented cell. (H) Dot plot for expression of C6 and MUC16 between FAS-rooted and basal-rooted clones within multiciliated clone extraction . The dot size represents the percentage of cells expressing the gene in the corresponding cluster, and the dot color indicates log-normalized expression level of the gene.

Article Snippet: The CellTag V1 plasmid library was purchased from Addgene (‘CellTag A’, Cat#124591).

Techniques: Transduction, Clone Assay, Sequencing, Labeling, Expressing, Derivative Assay, Extraction, Marker